Penicillin Amidase from Coliforms: its Extraction and some Characteristics

extraction and acetylation of purified trypsin from bovin pancreas and study of some its physico-chemical properties

آنزیم تریپسین در شرایط قلیایی ناپایدار می باشد .و فعالیت پروتئولیتیکی تریپسین منجربه خود هضمی آن در جایگاههای خاصی می گردد. بنابر این آنزیمی با ناپایداری بالا محسوب میگردد. در سالهای اخیر موفق شدند که با ایجاد تغیرات شیمیایی با اضافه کردن فلزات خاص ، کلسیم و یا عمل استیلاسیون منجر به افزایش پایداری آنزیم تریپسین گردند. مطالعات در حال حاضر نشان می دهد که تریپسین استیله شده فعالیت آنزیمی خود را ...

Purification and properties of penicillin amidase from Bacillus megaterium.

A penicillin amidase, obtained from the exogenous medium of a Bacillus megaterium culture, was purified approximately 96-fold by means of two cycles of adsorption on, and elution from, Celite, followed by a further fractionation on carboxymethylcellulose. On the basis of sedimentation centrifugation analysis, the final preparation was deemed to be homogeneous with an apparent molecular weight o...

Some characteristics of penicillin-resistant staphylococci.

Production and Partial Characterization of Penicillin Amidase Produced by Bacillus megaterium from Temple Puja Wastes

Temple puja wastes are released in water bodies or land creating severe environmental pollution and health hazards. The temple wastes extract was used to isolate the Bacillus megaterium for the production of enzyme penicillin amidase as it recorded the largest zone of activity. The result obtained showed that on nutrient agar medium the grown colonies were confirmed by gram staining as Bacillus...

Coliforms from hides and meat.

Coliform tests were performed on 85 hide and 75 meat samples. IMViC reactions were determined on isolates from positive confirmed and fecal tests, and strains other than Escherichia coli were identified. Strains typed as Aerobacter aerogenes types I and II were identified as Enterobacter cloacae (51.4%), Klebsiella pneumoniae (21.5%), Enterobacter aerogenes (15%), and Enterobacter liquefaciens,...